Device
Part:BBa_K2719009:Design
Designed by: Andrea Pamela Jimenez Tapia & Rodrigo Valencia Ocampo Group: iGEM18_TecCEM (2018-08-08)
Leptin Coding Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is composed by a T7-lac promoter (BBa_R0184), a RBS binding site, a leptin coding region and a double terminator. Also, it was optimized for being cloned in pSB1C3 and transformed in E. coli BL21 (DE3). This construct allows for an IPTG controlled expression of leptin.
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Figure 1. Diagram of BBa_K2719009, leptin expression device.
Source
Synthetic part.