Device

Part:BBa_K2719009:Design

Designed by: Andrea Pamela Jimenez Tapia & Rodrigo Valencia Ocampo   Group: iGEM18_TecCEM   (2018-08-08)


Leptin Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed by a T7-lac promoter (BBa_R0184), a RBS binding site, a leptin coding region and a double terminator. Also, it was optimized for being cloned in pSB1C3 and transformed in E. coli BL21 (DE3). This construct allows for an IPTG controlled expression of leptin.

"T--TecCEM--BBa_K2719009Diagram.png"

Figure 1. Diagram of BBa_K2719009, leptin expression device.

Source

Synthetic part.

References